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The advent of 454 sequences presents new challenges to assembly.
Homoeologous genomes in polyploids such as tef have high levels of sequence identity that present enormous challenges to assembly.
These datasets pose significant challenges to assembly algorithms and can, depending on the repeat complexity of the genome, lead to fragmented or incorrect assemblies.
As mentioned earlier, because of ancient genome duplication and complex yeast telomere structure, the telomeres of different chromosomes typically share highly similar repetitive regions, which poses challenges to assembly.
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The low turn-out was a challenge to assembly members.
Exogenously bolstering the proteostasis network increases cellular fitness in the face of challenges to ribosome assembly, demonstrating the direct contribution of orphan r-proteins to cellular phenotypes.
However, liberals continued to complain about the distribution of seats and have not withdrawn their 43 legal challenges to the assembly's constitutional legitimacy.
The presence of interspersed repeats and segmental duplications in eukaryotic genomes poses serious challenges to genome assembly.
While there has been impressive progress in mosquito transgenesis and mutagenesis in the past 20 years [ 3, 8, 12, 14– 22], the large size of the Ae. aegypti genome (~1.3 Gb) and large transposable element load (~47 %) present formidable challenges to genome assembly, physical mapping and annotation [ 23– 26].
There are additional challenges specific to assembly of RNA-Seq data.
The challenges to automatically generate assembly sequences using CAD models lie in intelligent reasoning and analysis of the modelled assembly data.
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