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Airway responsiveness to MCh challenge was determined using anesthetized, intubated and ventilated mice.
Protection from wt virus challenge was determined by measuring inhibition of wt viral replication in nasal washes and in lung tissue.
The end point of the challenge was determined by the pediatric pulmonologist as positive in 35/36 children.
The Δmax cortisol after corticotropin challenge was determined as a marker of adrenal gland reserve response by subtracting the cortisol baseline value from the 60 minutes cortisol value.
In order to analyse αT2ib function, NF-κB driven reporter gene activity in HEK293 cells overexpressing both mTLR2 and αT2ib upon TLR2 specific challenge was determined.
The mice were chow-fed and the transcriptome of three female mice from each BXD line after CCl4 challenge was determined with Affymetrix Mouse Gene 1.0 ST microarrays.
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"The biggest challenge was determining the right ratio of authenticity and playability," Astolfi said.
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The most important conceptual challenge was determining what would qualify as literature on frailty.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com