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That challenge was confirmed late in the evening.
The reduced expression of these genes in the resistant host, upon gall midge challenge, was confirmed by semi-quantitative RT-PCR (Fig. 4) and one of them, Prolifera, showed statistically significant down-regulation in RP-I as compared to RP-UI.
Cell response to hypoxic challenge was confirmed by means of gene expression analysis of the hypoxia-inducible gene ankyrin repeat domain 37 (asKRdescribedescribyd Benitaita et al. (2009) using TaqMan gene expression assay (Hs00699180_m1; also Applied Biosystems).
In addition, complete protection in vaccinated pigs against homologous challenge was confirmed with the absence of PRRS viral RNA in the serum on DPC 7. As shown in Figure 1(d), pigs vaccinated with MLV had efficiently cleared the VR-2332 challenge virus from the blood to undetectable levels.
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Primate C-peptide levels less than 0.9 ng/mL are considered sufficient to indicate a diabetic status; however, our group has established that diabetes is durable when at least a 75% reduction in C-peptide levels post STZ is recorded [ 35, 37] and a negative response (lack of C-peptide increase) after a challenge is confirmed [ 4, 37].
The challenge dose was confirmed retrospectively as described above for i.p. challenge.
The challenge dose was confirmed by plating serial dilutions of the inocula on blood agar.
The challenge dose was confirmed retrospectively by serial dilution and plating on blood agar.
The actual challenge dose was confirmed by plate count.
The targeted challenge dose was confirmed by back-titration of the inoculum on Vero cells.
Activation of an immune response in challenged individuals was confirmed by assessing antibacterial activity of the hemolymph.
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