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The families were challenge tested during April May, 2009.
The parentage of the challenge tested animals was checked when the linkage map was created [ 39].
In all, 1024 offspring belonging to 7 full-sibling families that were challenge tested as described above, were successfully genotyped.
The challenge tested fish were from the 2005 year class of the breeding company Aqua Gen AS.
The mapping families were provided by the breeding company Aqua Gen AS, and were used also for QTL mapping for resistance against the viral disease Infectious Pancreatic Necrosis (IPN) (Moen et al., in prep .. Hence, the offspring had been challenge tested for resistance to IPN.
The most susceptible and resistant 20% of animals from each of the challenge tested families were sampled for DNA extraction and a random set of these selected for genotyping giving an overall mean survival 12.19 ± 9.89 SD hours post A. hydrophila challenge.
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Intervention: Subjects underwent cough challenge testing with inhaled capsaicin.
Women ≥37 years old underwent clomiphene challenge testing.
All patients underwent standard spirometry and methacholine challenge testing.
The diagnosis is confirmed by serial pulmonary function testing or inhalational challenge testing.
These challenges tested outdoor students in the way the pilgrims Christian and Hopeful were challenged in Bunyan's account.
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