Sentence examples for challenge of ascertaining from inspiring English sources
To circumvent the challenge of ascertaining RNA expression in infected cells with waning levels of housekeeping genes, real-time PCR was performed using primers specific for the cPMLΔ5&6 transcripts along with primers that recognize all PML isoforms (primers derived from exon 3) to compare the expression of cPMLΔ5&6 to total PML transcripts during viral infection.
In part because of the challenge of ascertaining the true extent of transmission, health departments elsewhere have believed the SARS epidemics to be under control, only to find later that spread had occurred beyond what was recognized.
Determining when the line between cultural diffusion and cultural appropriation has been crossed presents several challenges, starting with the difficulty of ascertaining the difference between the two.
In the meantime, the old ABC of detection – "assume nothing, believe nobody, challenge everything" – remains the surest way of ascertaining the truth.
However, the administrative data used in this real-world study present challenges of accurately ascertaining variables of interest.
The challenge, however, in finding out who or why has less to do with an immaculate trail — rather it's a task of ascertaining who hated him the most.
'The exercise of ascertaining the facts was a serious one".
If properly utilized, there might be a possibility of ascertaining the truth of individual identity.
Pool sequencing is a relatively young variant of whole genome sequencing, and it is a challenge to ascertain candidate SNP assays for individual genotyping, which may limit the efficiency of replicating pooled sequence loci using individual genotyping [ 42].
However, the lack of a positive control the equivalent of the active copies for monoallelically silenced genes poses a technical challenge in ascertaining the presence of biallelically occluded genes.
However, the validity of the self-administered version of the IBHQ may be challenging to ascertain in this population, which might benefit from having the questionnaire administered by an interviewer.
