Shedding peaked on dpi 2 in the challenge group and dpi 6 in the exposure group.
The coefficient of variability of recStO2 was 25%and33%3% for the negative fluid challenge group and the positive fluid challenge group, respectively, with a statistically significant difference between groups (P = 0.0453).
Clinical signs, including sneezing, nasal discharge, and coughing, were observed 2 8 dpi in the challenge group and 5 8 dpi in the exposure group.
Six animals from the challenge group and 2 animals from the control group were classified as slightly affected (Table 3 c, d).
Incorporation of a nonsensitized challenge group and use of smaller non-irritating exposure doses facilitate distinguishing irritants from sensitizers (Karol, 1994).
On day 21, consolidations and ground glass opacities were observed in 14 animals in the challenge group and also in 2 pigs in the control group (Table 1, Figure 2a, 2b).
After 1 week of acclimation, the rats were randomly divided into a control group and a challenge group, and they were fed a normal pellet diet (NPD) consisting of 10.3% fat, 24.2% protein and 65.5% carbohydrate and a HFD consisting of 59.8% fat (mainly pork), 20.1% protein and 20.1% carbohydrate.
Exoskeleton thickness increased in both sham-challenge groups and the 1N group but decreased in the 2N group.
Pearson test revealed no significant correlation between the extent of sensitization (as measured by the change in mean velocity between acute and challenged groups) and the extent of reinstatement (r = −0.33, p>0.05) (see figure 7a).
To test what the effects of missing such a case would be, a sensitivity analysis was performed by increasing the positive responses in the challenge groups (infection and illness) by one (scenario 1) or by two individuals (scenario 2).
