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This is a serious challenge for study of these regions, and also demonstrates the importance of using haploid material for study of this type of regions.
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A challenge for studying the poly A- transcripts is to distinguish the true poly A- transcripts from the degraded transcripts originated from in vivo physiological RNA metabolism or in vitro RNase activities (which also lack the 3' poly A tail).
Understanding the genetic variation of phenotypic responses to a range of environments (referred to as phenotypic plasticity or reaction norm) and its impact on selection and evolution has been a central challenge for studies in evolutionary genetics and ecology [1] [3].
A major challenge for studies such as ours and those that investigate the interaction between thousands of variants is to decipher the complexity of the genetic networks between the loci that appear to have a statistical relationship [44].
This continues to be a major challenge for studies of ecological and evolutionary genomics [ 6].
A major challenge for studies in contemporary evolution is the role of epigenetic inheritance.
The lack of a true placebo condition for visible light interventions is the greatest challenge for studying the effects of bright light therapy [ 57, 73, 74].
As already mentioned in the Introduction section, a further challenge for studies attempting to employ ecologically valid self-paced reading paradigms is a certain loss of experimental control.
A challenge for studies in the field of physical activity and health is to encourage the use of standard instruments and analysis strategies which enable more comparison between studies and pooled conclusions.
19 A universal challenge for studies that seek to explore the impact of social disadvantage in society is how to best capture the concept of 'social disadvantage' and define socioeconomic position.
The difficulty of disentangling the confounding effects of secondary dispersal from the impact of natural historical processes presents a persistent challenge for studies on the historical biogeography, particularly of species prone to intentional translocation by humans.
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