Challenge of differentiated cells with either 1 μ m 27-OH or 1 μ m 24-OH was, in fact, the only experimental condition consistently showing a very strong enhancement of toxic Aβ production (Fig. S1).
The challenge of differentiated SK-N-BE human neuroblastoma cells with patho-physiologically relevant amounts of 27-OH and 24-OH showed that both oxysterols induce a net synthesis of Aβ1-42 by up-regulating expression levels of amyloid precursor protein and β-secretase, as well as the β-secretase activity.
The essays below describe how public health workers engage with similar challenges and differentiated opportunities resulting from the disinvestment of the state in systems of healthcare and sanitation – from privatization and NGO-ization, to transnational research and forms of political performance.
The third and most difficult challenge is this: differentiated tuition fees, whereby students from poorer families pay nothing and those from wealthy backgrounds pay perhaps £10,000 a year or a bit more.
The analysis of centromeres and telomeres by proteomics has been a particular challenge, as they are differentiated regions of the single long chromosomal DNA molecule, rather than independent structures in their own right.
Pathologic distinction of medulloblastoma from pineoblastoma can occasionally pose a clinical challenge given that these poorly differentiated tumors are morphologically indistinguishable and larger tumors may grow to involve both the superior cerebellum and pineal regions.
Furthermore, for future studies, we recommend to include more than two different dilution steps for the titrated nasal challenge test to obtain more differentiated effects.
Numbers need to grow, and distribution is also a challenge, suggesting the need for differentiated policies in relation to rural areas, especially for doctors and specialists.
Probably, individuals seeking to show they are assertive or challenging can be perceived as differentiated or imposing figures.
The paradigm that all cardiomyocytes are terminally differentiated has been challenged.
