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Responses were long lasting (6 months), boostable by a fourth MVA vaccination and in vivo cross-reactive as demonstrated in a surrogate Listeria-based challenge assay.
Real time PCR studies and the glucose challenge assay have shown that cells on GPE scaffolds could express and secrete insulin and glucagon in vitro.
In order to develop live vaccine against Siniperca chuatsi rhabdovirus (SCRV) disease, an avirulent virus strain, designed as Micropterus salmoides rhabdovirus Sanshui (MSRV-SS), was selected from six fish rhabdovirus isolates (SCRV-QY、SCRV-SS、SCRV-GM、CMRV-FS、OMBRV-JM、MSRV-SS) by fish challenge assay.
More important, EVETPIRN-peptide (aa6-13) conjugated immunogen induced high M2e specific antibody titer (1 25,600) in mice after booster immunization, and provided mice significantly 40% higher survival rate compared with the control group in challenge assay (p=0.0215), which suggested the EVETPIRN-epitope was immunogenic and actively conferred immune protection to some extent.
The challenge assay was performed for 24 h and culture supernatant was then collected.
The results of the challenge assay support the previous reporter knockdown data that lhRNA knockdown efficacy is primarily mediated by first position siRNA sequences located at the base of the duplex stem.
Similar(34)
Different pandemic virus strains were used in HI, microneutralization and challenge assays according to their availability.
Using our cytogenetic challenge assays, we conducted an investigation to address the deficiency.
Virus challenge assays were performed by Agrobacterium-mediated vacuum infiltration [ 35].
Moreover, in challenge assays phi-IPLA5 had lytic capability against S. epidermidis[ 15].
The in vitro challenge assays revealed two bacterial strains that inhibited B. dendrobatidis: Citrobacter sp. KC853149 and Brevundimonas sp. KC853150.
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