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It is the UK's fourth-largest supermarket chain, trailing Tesco, Asda and Sainsbury's in annual sales.
Now the big U.S. retailer will be Brazil's third-largest chain (trailing only CBD and France's Carrefour), with $1.6 billion in sales.
The ensemble stood in a row, canonically changing positions until one broke the chain, trailing off into a développé effacé, her leg soaring above.
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We have designed an analogous single polypeptide chain TRAIL variant (scTRAIL), which was used to construct a scFv scTRAIL fusion protein for tumor targeting.
Twenty-two yearlierrlier, he co-founded Home Depot with his friend Bernard Marcus, which they built into a retail chain that trailed only mighty Wal-Mart in revenue.
The chain now trails only Home Depot and $7 billion revenue Lowe's in the $140 billion (retail sales) home improvement industry.
Apoptosis induction by a single-chain TRAIL (scTRAIL) molecule could be enhanced >10-fold by generation of epidermal growth factor receptor (EGFR -specific scFv-scTRAIL fusion protEGFR -specific
In support of this, we have recently shown that a fusion protein comprising scFv and single-chain TRAIL is a preferred format compared with nontargeted TRAIL in terms of bioactivity in vitro and in vivo.
Our previous results showed that three extracellular domains of human TRAIL can be fused by peptide linkers to yield highly active single-chain TRAIL (scTRAIL) that can be further endowed with target-dependent activity by fusion to an antibody derivative.
To generate a single-chain TRAIL molecule, we followed the design principle described for scTNF and covalently fused three TRAIL monomers, each consisting of the extracellular domain of TRAIL (aa 95 281) through two peptide linkers comprising four repeats of the sequence GGGS.
To test the effect of disallowing torsional motion of the end, for huco2, we applied restraints to three Cα atoms of residues 4, 3, and 2, respectively, from the leading, middle, and trailing chain, and likewise restrained residues 28, 27, and 26.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com