Sentence examples for chain shuffling from inspiring English sources

Chain shuffling of the heavy and light chains provided the best binders.

Since antibodies are dimeric, simultaneous diversification of heavy and light chains on separate genetic elements would allow "chain shuffling" to occur simply and efficiently.

The new recombinant clones of Fab that were phage-enhanced using chain shuffling were candidate molecules for investigating molecular mechanisms of SDM antigens specific binding and applications.

Light (L) chain shuffling is routinely used to analyze optimal L chains that pair with a specific heavy (Fd or H) chain, which ultimately leads to in vitro affinity maturation of a particular antibody.

The ability to express antibody heavy and light chains from separate but compatible high copy plasmids should allow new opportunities in antibody engineering, such as rapid chain shuffling and generation of more complex antibody libraries.

Herein, we designed of the dual-vector system-III (DVS-III), which is composed of a set of pLf1T-3 phandmid and pHg3A-3 plasmid, for L chain shuffling of any given human Fab antibody via phage display technology.

The A8 clone previously obtained from an original phage antibody library was further affinity-matured by light- and high-chain shuffling respectively, to generate the end product B9 clone.

Two unique clones were selected for further affinity improvement by light-chain shuffling.

This clone was affinity-matured using a light-chain shuffling method and rescreened with rFRβ under a more stringent set of conditions.

The antibody derivatives were generated using a chain-shuffling approach based on human antibody variable gene phage-display libraries.

We have generated human derivatives of the murine Guy's 13 antibody using a chain-shuffling approach based on human antibody variable gene phage-display libraries.