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Single step processes involving radical-mediated addition of PP to triallyl phosphate are shown to generate bimodal products with highly differentiated chain populations, while a two step sequence involving PP addition to vinyltriethoxysilane followed by moisture-curing is shown to generate more uniform architectures.
These HS chain populations are then evaluated for structural patterns of domain organization.
As demonstrated with HS chain populations from two different cell culture sources, the model has the ability to predict significant differences in overall domain organization properties as well as in the density and distribution of specific functional motifs.
Thus, differences in the biological activities of various HS chain populations, including HS isolated from diseased and nondiseased tissues, can be correlated to differences in either the overall domain organization or the presence of specific structural motifs within the population.
We harvested nascent chain populations of wild-type and ΔF508 CFTR after 20 and 30 min of synthesis, as well as full-length proteins (60 min) (Figure 5A), and subjected them to treatment with 5 µg/ml of Proteinase K (Figure 5B).
Almagro et al. [ 50] have reported the domination of these canonical classes within sheep heavy chain populations.
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It is, however, not possible to determine whether the recorded change in the acyl chain is because of alterations in the triglyceride, cholesterol ester or ether-linked fatty acyl chain population, or a mixture thereof.
While macroradical fragmentation reduces the molar mass of the dominant chain population, cross-linking by triallyl trimesate (TAM) activation yields a minority population of hyper-branched chains that is less susceptible to molecular weight loss.
Low-frequency dynamic shear viscosities as well as extensional viscosities are shown to be highly responsive to a hyper-branched chain population, whose abundance and molecular weight correlate with the kinetic chain length of the grafting process, and the propensity of a coagent to oligomerize.
B-cell immunoglobulin gene rearrangement and T-cell receptor gene rearrangement studies using the polymerase chain reaction method indicated that a monoclonal immunoglobulin kappa light chain population was present and that the T-cell population was not monoclonal.
As the reaction progresses, the amount of branching doubles, reaching 7% of the total chain population by 4 h.
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