The left panel shows a scatter plot of the mean nFret vs Cfp values in individual cells.
The RFP was attached to a chromatin protein to mark the nucleus and the RFP value was used to normalise the YFP and CFP values on a per-cell basis to control against imaging variations.
Money talks and more playoff contests would most likely increase the CFPs value to more than $1 billion per year vastly exceeding the current cash cow king, the NCAA men' s Final Four basketball tournament, which is currently petering along at a mere $700 million per year.
'Effective total ligand' refers to the ligand availability observed at a given ligand-CFP fluorescence value in a cell line expressing only ligand.
The resulting ESAT-6 and CFP-10 values were summated to give the total RD1 SFC count.
Using this background subtraction the values for CFP fluorochrome in the YFP channel and YFP fluorochrome in the CFP channel were effectively zero.
It is interesting to note that, although the cells expressing myrGag CFP show a lifetime that is consistent with previously reported values for CFP, the cells expressing the normal Gag CFP construct show a reduced lifetime, where the majority of their fluorescence is observed at the membrane.
According to the Resolution 002/2003 of the Brazilian Federal Council of Psychology (CFP, 2003), the minimal acceptable value for this index is.60.60
The average, maximum and minimum pore diameters and pore-diameter distributions in the track-etched membranes were obtained by CFP, and compared with the values obtained by different techniques like atomic force microscopy (AFM), scanning electron microscopy (SEM) and molecular weight cut-off (MWCO).
This gives the raw fluorescence values for YFP, CFP and RFP (yellow, cyan and red fluorescent proteins, respectively) for about 400 to 500 cells for each variant under different doxycycline concentrations.
The activity of RhoA is monitored by measuring CFP (480 nm)/YFP 545) fluorescence emission values upon excitation of the transfected cells at 430 nm.
