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These lines were established to provide (1) the line of the occlusal plane, established by the occlusal surfaces of the lower first and second molars; (2) the cervical line, delineated by the cervical bone limit of the lower second molar; (3) the line of the lower margin of the ascending mandibular ramus; and (4) the longitudinal axis of the M3.
Previously, two studies [1, 24] used complete root formation with the highest part of third molar below the cervical line of second molar as a criterion for third molar impaction, but they do not consider the direction of eruption that is likely directly related to the impaction potential.
Cells from the HeLa human cervical line were previously cultured in Eagle's minimum essential medium (MEM, PPA Laboratories, Germany) supplemented with 10% fetal bovine serum (FBS, Laborclin, Brazil), 0.1 mM non-essential amino acids, and 1 mM sodium pyruvate, at 37°C in a humid oven with 5% CO2.
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If the cervical lining was smooth, uniform, with no acetowhite lesion the test was classified as negative [ 12].
Despite best practice evidence favouring cervical lines, the study found that femoral line insertion was more appropriate in this subset of patients with unstable head injuries.
Another study comparing the leakage of Biodentine with a resin modified glass ionomer (Fuji II LC) was one by Raskin et al. [ 34] where silver penetration was evaluated in cervical lining restorations.
After disinfection of the surgical site under local anesthesia, we have performed a palatal incision along the cervical lines from tooth 15 to 25, using a scalpel with a no.15 blade.
The four promising compounds (18, 26, 28 and 33) were tested for safety through cytotoxic assay against human cervical cell line (HeLa) and compatibility with vaginal flora, Lactobacillus.
All the synthesized compounds were assessed for their safety through cytotoxic assay against human cervical cell line (HeLa) and compatibility with vaginal flora, Lactobacillus.
d Schema of the tracheoesophageal fistula Fig. 4 a Cervical incision line.
The normal human cervical cell line (CRL2614) and malignant human cervical carcinoma cells (CaSki) were used in this study.
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