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The analyzer was calibrated regularly using a certified calibration gas (Linde AG, Munich, Germany).
Oxygen and CO2 analyzers were calibrated with room air and certified calibration gases at 180 kPa (16% O2, 5% CO2 and 79%N2).
Certified calibration solution standards purchased from Sigma Aldrich and NRC-CNRC (Certified Reference Materials Program, Institute for Marine Biosciences, National Research Council of Canada) were used for method development, analyte identification and quantitation.
The certified calibration gas cylinders for each system came from the same production run (Lot ZP12Y161BB) and used identical Victor CGA973 pressure regulators (Denton, TX).
Trained researchers assessed accuracy and consistency using certified calibration weights at 10 kg, 25 kg, 50 kg, 75 kg, 100 kg, and 110 kg.
Calibration of flow was performed using a certified 3 L calibrated syringe and calibration of the O2 and CO2 gas analysers was performed prior to each experimental session using a certified calibration gas.
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Prior to testing, calibration with certified weights was used to established equations to derive force from the pressure recordings.
External calibration utilizing certified reference materials (CRMs) which match the composition of the sample to be investigated to the largest possible extent preferably exactly is the most reliable method for accurate quantification in LA-ICP-MS [ 20, 22– 22].
O2 and CO2 sensors were calibrated prior to each test using high-grade calibration gas with certified gas concentrations (O2 = 16%, CO2 = 5%, N2 = balance).
Laser induced breakdown spectroscopy (LIBS) has been evaluated for the determination of micronutrients (B, Cu, Fe, Mn and Zn) in pellets of plant materials, using NIST, BCR and GBW biological certified reference materials for analytical calibration.
This category of biomarker assay lacks calibration against a certified standard, but reports numerical values as a characteristic of the sample.
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