Because fewer subjects than planned were studied and the statistical outcome was not conclusive, these results certainly need replication.
Although we do not know which viral factors are necessary for the high pathogenicity in mice, the efficiency of viral replication certainly is one likely determinant.
Thus, the current result is far from genome-wide statistical significance and certainly requires independent replication.
However, statistical confidence in protein identification certainly increases through replication and independent identification of the same proteins [ 25, 26].
It is certainly possible that replication has initiated at these sites but the extent of EdU incorporation was not yet sufficient to generate a detectable signal.
Even though the absence or presence of association in a given population is dependent on many factors (e.g., number of individuals, ethnicity, SNP prevalence, BMI, familial history of T2D and others), replication studies certainly help in validating SNPs truly associated with T2D and in excluding false positives.
Although each ecological and evolutionary study is unique and thus strictly speaking cannot be proven wrong through replication, this certainly does not make replication to assess validity of an earlier finding a waste of time.
The most significant matches were ORFs 16, 31, 35, which are almost certainly the origin of replication complex, integrase and helicase functions respectively of the virus, having highly significant matches to full length proteins in Har.
Here, the identification of a polymorphism is a axon guidance pathway gene is intriguing, but certainly requires independent corroboration and replication before any strong claim can be made.
They are always materializing as an unavoidable consequence of a replication mechanism that certainly must have its error rate.
