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We measured the sensitivity, specificity and MCC at certain stringency thresholds.
When applying certain stringency thresholds, it also significantly outperforms PSI-BLAST and BLAST at nearly all of the thresholds (Table 2).
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Under conditions of reduced stringency, certain lanes exhibited more than the two expected bands (data not shown), indicating the existence of related sequences within the genome.
Considering a certain level of stringency in our parameters we still have found AS evidence in 44.6 % of intron-containing genes, slightly more than the 30 % recently reported [ 37].
However, these effects turn negative once the level of stringency leaps over a certain threshold.
Furthermore, we improved the stringency of the prediction by considering certain quantitative scores.
An increase in the stringency of the confidence criterion beyond a certain point does not increase accuracy, and only excludes more of the data.
A nice feature of the algorithm is that probes can be assigned to each distribution with a certain probability, allowing us to increase or reduce the stringency of our assignments easily.
The budgetary stringency is real.
Yet stringency is just what is needed.
The adviser says stringency is now unavoidable.
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CEO of Professional Science Editing for Scientists @ prosciediting.com