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We speculate that certain checkpoint proteins, and perhaps the Cdc20 effector, not only undergo structural conformational change [19], but also receive post-translational modification when associated with kinetochores.
DNA replication, as measured by 5-ethynyl-2′-deoxyuridine (EdU) incorporation, was partially recovered in RB-KD OIS cells, probably from the activation of certain checkpoint pathways (Fig. S1E) (Chicas et al., 2010).
We also substituted a gel in which the +HU lanes are more equally loaded to compensate for the ∼2-fold reduction in DNA due to the lack of DNA replication in cells treated with HU. Figure 4 was intended to refer to the decrease in DSB formation that occurs in certain checkpoint mutants in the absence of checkpoint activation.
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All flights over the ocean cross certain checkpoints, and the most critical one for Flight 447 was Tasil Point.
The university enlisted the help of a third party contractor, he explains, who implemented certain checkpoints that would catch and resolve possible concerns.
Therefore, a cell cycle control system can arrest the cycle at certain checkpoints.
Perhaps certain checkpoints involved in substrate detection, recognition, and unfolding are bypassed, allowing faster retrotranslocation to occur.
Cell-cycle arrest is known to occur at certain checkpoints when DNA is damaged, and modification of the cell cycle is an important index to measure when monitoring radiation-induced responses.
Feel free to dangle smaller rewards in his face at certain checkpoints.
You will be flying a course that flies you over Los Santos and back to the airport, but you will be performing stunts at certain checkpoints.
If you know the assignment is going to take a while, then set certain "checkpoints" that when you complete them, you get a reward of some goof-off time or whatever works for you personally.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com