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Subject numbers were generally between 8 and 10 for each exposure/assay but may have varied from as few as 5 to as many as 14 for certain assays.
However, we like to mention that we are not fully convinced by the statement that the differences in technical formats (blotting vs. pull-down) is the only or main reason of differences in antibody performance between certain assays.
Conversely the limitations also provide some advantages in that our results are a stronger validation of the intrinsic value of the identified biomarkers because the biomarker diagnostic potential is preferably not limited to just certain assays.
Earlier it had been shown that in certain assays E. coli and B. subtilis can elicit characteristic immune gene expression responses in Drosophila [28].
Alternatively, either of the two other HIV vaccines evaluated in the current study may have the potential to provide even better protection than the canarypox prime-recombinant Env protein boost approach if the higher responses in certain assays observed only in the HVTN 041 or DP6-001 trial sera are any indication.
In the longer term, simulation offers the hope of replacing certain assays, and suggesting novel ones with greater predictive power.
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And when they knocked at the door, they weren't quizzed about GMP (good manufacturing practices) or about how to run a certain assay a certain way.
NPs have been described to interfere with assays, and some reviews report the limitations of certain assay systems [36] and that AuNPs even have the capacity to quench or enhance fluorescence depending on the plasmon field and dipole energy [37].
Certain assay variations have immobilized ConA to a solid-phase (microdialysis membranes, beads, etc).
If water runs were undetected for a certain assay, the lowest value of all detected water runs was used.
Even though manufacturers provide clear directions on how to optimally use their kit and perform a certain assay, some laboratories adapt kits to their own protocols [ 12].
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com