Brain resections were collected in an ice-cold artificial cerebrospinal fluid solution bubbled with 5%CO2and95%5% O2.
Perfusion fluid for microdialysis was a modified artificial cerebrospinal fluid solution previously described by Moghaddam et al. (12).
In a few experiments, the bicarbonate-based artificial cerebrospinal saline solution (137 mM NaCl, 2.5 mM KCl, 2 mM CaCl2, 1 mM MgSO4, 12 mM NaHCO3, 1 mM Na2HPO4, and 11 mM glucose, pH = 7.4, equilibrated with a mixture of 95%O2-5O2-5%2) was used to dissect and study neuromuscular transmission from both wild-type and ASIC1a −/− mice.
Horizontal combined entorhinal cortex-hippocampus slices (350 μm) were prepared with a Leica vibratome (Leica VT1200S) and were then stored in an interface chamber that contained artificial cerebrospinal fluid solution (aCSF) containing 119 mM NaCl, 2.5 mM KCl, 4 mM MgSO4, 4 mM CaCl2, 26.2 mM NaHCO3, 1.0 mM NaH2PO4, 11 mM glucose, and gassed with 95%O2/5%% CO2.
A cerebrospinal fluid-like solution, infused through this catheter, allows hourly sampling of patients' brain extracellular fluid into microvials for bedside analysis [56].
Slice cultures on membrane inserts were transferred to a submersion-type recording chamber continually perfused with 30 32°C oxygenated (95% O2 5% CO2) artificial cerebrospinal fluid (aCSF) solution containing (in mM): 117 NaCl, 4.7 KCl, 2.5 CaCl2, 1.2 MgCl25 25 NaHCO3, 1.2 NandPO4 and 11 glucose.
A cerebrospinal fluid-like solution, infused through this catheter, allows hourly sampling of patients' brain extracellular fluid into microvials for bedside analysis [ 56].
The collections of microdialysis perfusates were then initiated approximately 3 h after inserting the probe and circulating an artificial cerebrospinal fluid (CSF) solution (in m m: NaCl, 145; KCl, 2.7; MgCl2, 1.0; CaCl2, 1.2; containing 0. m m ascorbate and pH adjusted to 7.3 7.4 with 2 m m sodium phosphate buffer) through the probe at a flow rate of 0.8 μL/min.
After confirmation of clear cerebrospinal fluid (CSF) efflux, solution A (n =60) or solution B (n =60) was administered.
The miniature fuel cell, shaped very similarly to a computer chip, was tested in a saline solution simulating cerebrospinal fluid, at sizes of 1 square millimeter and 2 square millimeters.
