The second prevalent cerebellar cell type is the granule cell.
Furthermore, unbiased gene expression analyses revealed only partial overlap in the cerebellar cell genes and pathways that were altered by the Cln3Δex7/8 and Cln6nclf mutations.
In other sets of experiments, Green E17 cerebellar cell suspensions were co-cultivated with GCs (E17+GC), which were obtained from P6 Wistar pups following pubished protocols [47], [48].
Importantly, with the exception of oligodendrocytes no other cerebellar cell types appeared affected by the treatment in MBP-TK mice, as verified by the use of cell type specific markers (Figure S1).
Since it is known in mice that Sox2 is expressed in the nuclei of Bergmann glia [28] we used the marker Brain Lipid Binding Protein (BLBP) to visualize cell bodies of Bergmann glia, in order to further investigate this radial glia-derived cerebellar cell type in rabbits.
Therefore the declining influence of isthmic FGF8 is permissive for the generation of cerebellar cell types.
(A ) The developmental lineages of the major cerebellar cell types are illustrated.
During development, major cerebellar cell populations are derived from two germinal zones.
