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Although data was generated from a single centre, comparison with data from other primary care studies suggests that the participants in our study are representative of the patients with back pain in primary care [ 7, 41].
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The performance and quality control of the PET/CT devices were monitored by using a specific phantom developed by our group [29], and the post-injection delay was kept constant to facilitate inter-patient and inter-centre comparison.
There is also a need for larger multi-centre studies for cross-centre comparison.
Data from 4537 patients were converted to Quality and Outcome Framework (QOF) scores to enable inter-centre comparison.
Notwithstanding the limitations of a non-randomized single-centre comparison, the results are in line with the very preliminary data of randomized comparisons, mentioned above.
Mantel-Haenszel (MH) is a within-centre comparison, where the treatment effect is calculated within each centre and then combined for an overall effect [ 6].
However, up until recently, cross-centre comparison of migration data was challenged by the lack of a standard definition of migration in HDSS centres, e.g. varying threshold cut-offs used for defining migration (from 1 to 6 months).
With exception of CTX-M, which was not tested, or partially tested as is shown in Table 1, results of the multi-centre comparison were all concordant and again, the results were 100% concordant with listed strain characteristics.
The experts used to annotate the EEG recordings for seizure are all from a single centre; although the level of agreement for these short recording is comparable to multi-centre comparisons for the annotation of neonatal EEG seizures28.
Due to the small sample size and different proportions of emergency patients, relevant between-centre comparisons cannot be made.
A similar issue was also identified regarding the proportion of respondents who identified an affiliation with a CCLG unit, which may have been due to the relatively large number of centres in comparison to TCT centres at the time of the study.
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