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While blocks were formalin-fixed and paraffin-embedded in different anatomical pathology laboratories, they were centrally extracted for DNA utilising the same protocol, and run through the same MPS platform (Illumina TruSeq Amplicon Cancer Panel).
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Since the resolution of their data is significantly lower than the training dataset used in the FRM, we extracted centrally located CpG as a stand-in for each region.
Data from individual practices were extracted centrally via a bespoke clinical reporting query established with the participating practices and groups.
Gene expression profiling was carried out on whole blood RNA extracted centrally by qLAB using the PAXgene 96 blood RNA Kit (Qiagen) at baseline: 67 samples were available for GHD and 44 for TS.
In order to explore possible mechanisms related to the GHRd3-GHD severity association, GE profiling was performed at baseline on whole blood RNA extracted centrally by qLAB using the PAXgene 96 blood RNA kit (Qiagen).
Genotyping was performed centrally on DNA extracted from whole blood using an Illumina GoldenGate micro array, containing 1536 single nucleotide polymorphisms (SNPs) located in 103 candidate genes related to i) the GH/IGF1 axis, ii) bone and cell growth and iii) glucose and lipid metabolisms.
Using the 158 IHC centrally confirmed patient samples, we extracted RNA and profiled the samples using a previously reported 96-gene BioMark™ Fluidigm microfluidics quantitative RT-PCR-based RT-PCR-based RT-PCR-baseded to caplatformpression of breasthatncer genes involved in proliferation and various aspects of breast cancer signaling [ 16].
Clinicopathological data were extracted from the medical charts and centrally reviewed for all patients.
Equation (2) and Table 1 used in the present study are extracted by Ince (2012) from the numerical results based on centrally cracked finite strip with a finite width specimen.
Now that the data is held centrally, GMFRS is looking to extract intelligence from it.
Continuous EEG monitoring is performed for 48 h after inclusion, and a 30-min EEG extract is interpreted centrally before implementation of the randomly allocated treatments.
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