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The pathophysiology as well as the peripheral or central localization of this deficit is unclear.
A 3D representation of the confocal data (Movie S1) nicely illustrates the central localization of Nuc within the neutrophil, consistent with its Golgi and ER localization.
In parallel we observed central localization of cells expressing cleaved caspase-3 on day 12 of 3D culture.
DOI: http://dx.doi.org/10.7554/eLife.01149.008 10.7554/eLiFigure49.009 Figure 2 figure supplement 2. STORM image of Integrin β1 and Laminin β2 confirms the central localization of Laminin.
This implies that ARF6 activation results in a more central localization of TLN within the dendritic shaft, suggesting an increased internalization.
Nup98 shows by post-embedding immuno-EM a very central localization within the central translocation channel of the NPC (Krull et al, 2004).
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However, the following pieces of evidence do to some extent suggest central spindle localization of the CPC to be important.
Since waist circumference (WC) is strongly correlated with central fat localization [ 3], it is a sensitive measure of central-type obesity.
HAL-2, a recently discovered C. elegans nuclear protein, was found to prevent premature SC central element localization to unpaired chromosomes.
We speculate that the down-regulated MEF2C gene expression might play a role in the progressive central nucleus localization observed in the skeletal muscles of Dox-treated Tg(HQK) mice [ 7] through a reduction of the Srpk3 activity.
All other proteins were disposed in ring-like patterns around the centriole and, as shown above for γ-Tubulin and NEDD1, CPAP showed both ring and central dot localization (Fig. 1A, Fig. 2A).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com