Exact(10)
The center of the stack was determined by identifying the slice of maximum phase value [ 21].
An image was selected from the center of the stack to show the distribution of HBD3TAMRA within the cell.
In addition, an XZ image through the center of the stack is shown in the same figure (top left).
Then, puncta densities (number of puncta per µm) were calculated in increasing eccentric volumes from the center of the stack where the NG2 cell soma was located.
A structured image was acquired near the center of the stack, where there were multiple in-plane and out-of-plane beads at various depths throughout the PAA gel.
However, the unique cisternal continuity detected in this cryo-tomogram was also very different from other luminal continuities between adjacent cisternae as described during synchronized viral cargo waves (Trucco et al. 2004), as these were located at the outermost rim of medial cisternae, but not in the center of the stack.
Similar(50)
For electron tomography, the analysis of intercisternal connections in chemically and cryo-fixed samples was performed on 200-nm-thick sections, passing roughly perpendicularly to the center of the Golgi stack, as described previously (Trucco et al., 2004).
Boutons labeled with a terminal type-specific marker were selected in a single-channel image obtained from the center of the optical stack (approximately 5 μm from the surface of the section), and analysed by Adobe Photoshop CS5.
Begin sewing from the bottom up (so that the knot stays on the bottom of the flower) and sew together in the center of the flower stack.
To further ensure that the total dry mass is captured over the entire depth of the cell, the projected maximum of the 5 slices around the center of the z-stack is used to calculate the dry mass density map.
Logs were stacked, and the woman was placed in the center of the pile.
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