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The per cent of cells in different phases of the cell cycle was determined using the Multycicle software version 2.53.
Twenty-six per cent of cells in this population were G0 cells compared with 2% in exponentially growing cells.
The program determines the per cent of cells within each peak, and the sum of all peaks in the control culture is taken as 100% of proliferation and 0% of suppression.
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They do the scratchy high-in-the-throat drawl, the run-on pronouncements studded with arcane facts and statistics ("And with every ten per cent of cell-phone penetration G.D.P. goes up point six per cent "), and the trademark exclamations ("Isn't that fascinating?").
The number of apoptotic cells was expressed as a per cent of total cells counted.
The results are expressed as the per cent of migrated cells as compared with the control (untreated cells).
At least 300 cells were scored for the incidence of apoptosis, in five randomly selected fields and the per cent of apoptotic cells with fragmented nuclei and condensed chromatin relative to the total was calculated.
Apoptotic cells were examined at 400× magnification, counted in 10 randomly selected fields, and expressed as a per cent of total cells.
"It turns out that some small per cent of tumor cells are actually the deadly ones," he went on.
At the start of each day, as many as thirty per cent of the cells could differ from the previous session.
He said, "The point of the paper was that with any severe, sub-lethal stress — lethal enough that it kills, say, seventy or eighty per cent of the cells — the surviving ones will convert.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com