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Third, for Cel5A-CBM the cellulose-buffer interface moved to greater depths, indicating film expansion at RT.
The substrate 4-methylumbelliferyl-β-D-cellobioside was incubated for 1 hr with purified LYS1, commercial reference cellulose, or buffer as control.
CBM: Carbohydrate-binding module; GH: Glycoside hydrolase; DNS: Dinitrosalicylic acid; PASC: Phosphoric acid swollen cellulose; TBS: Tris buffer saline.
To analyze in detail the effect of recombinant swollenin on cellulose, different cellulosic substrates were pretreated with buffer, BSA or recombinant swollenin.
Leaf strips were digested in a buffer containing cellulose R-10 and macerozyme R-10.
We assume that amorphous cellulose in an aqueous buffer will behave as a glassy polymer.
The biotin positive control was created by using 7.5% of the DMSO peptide stock solution with 67.5% DMSO in addition to the Cy3 cellulose conjugate and SSC buffer.
Extracted dried pigment pellet was subjected to the dialysis in cellulose membrane against phosphate buffer of pH 7.0 and purified by column using Silica Gel material of 60 120 mesh size.
The crude laccase extract was further purified by three successive steps of ion exchange chromatography: firstly on DEAE-cellulose (10 mM phosphate buffer, pH 7.0) with a flow rate of 2 mL/min, secondly on CM-cellulose (10 mM phosphate buffer, pH 6.6) with a flow rate of 2 mL/min, and finally on Q-Sepharose (10 mM sodium acetate buffer, pH 4.0) with a flow rate of 1 mL/min.
A culture broth sample of 20 μl was mixed with 20 μl of 2% Azo-CM-Cellulose in 2× McIlvaine Buffer (McIlvaine 1921) at the desired pH.
Vehicle (0.3% (weight/volume) Hydroxypropyl cellulose (Sigma) in Phosphate Buffered Saline (1 ×PBS, Corning)) was filter sterilized and was also administered by i.p. injection (100 μl per injection) in vehicle control mice.
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