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Interestingly, the cellular yields between the two lines were not significantly different, despite significant differences in growth rate (data not shown) and substrate consumption.
The cellular yields and the functional quality of the DC was comparable in all the experiments that were performed, indicating that no disease-specific factors had influenced the BM quality and thereby modulate DC function.
However, cytological interpretation can be problematic; low cellular yields, atypia, degenerative changes, urinary tract infections, stones and intravesical instillations hamper a correct diagnosis.
For one, cellular yields are inconsequential when using stem cells due to the ability to scale undifferentiated cell populations, whereas primary embryonic sources of endothelial cells and particularly BMECs are nearly impossible to obtain in significant amounts.
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Day 15 was chosen for sampling based on cellular yield plateau seen in previous experiments (data not shown).
The major limit for its success is represented by the low cellular yield of the stem cell population.
Irrespective of shake flask mass transfer implications, the elevated substrate consumption did not generally lead to any enhancement of cellular yield or TE formation.
Changes to flask volume and cell line were observed to modify substrate carbon consumption within the cell culture, whilst having no significant impact on overall cellular yield.
We conclude that by increasing the extent and frequency of the reciprocatory motions used by an experienced sampling operator as well as enhancing the ejection pressure, the cellular yield can be increased almost three folded compared to the standard protocol.
Noting differences between in vitro and in vivo data, in general, optimal expression patterns concerning chondrogenic differentiation were achieved by few passages, medium cellular yield, short membrane-holding time.
When choosing a line for commercial scale up, cellular yield is an important measure to consider.
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