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The decrease in blood flow with tumour size was also apparent in densely cellular, viable tumour regions when analysed separately.
However, the peripheral to central gradient of blood flow was apparent even when densely cellular, viable tumour regions and necrotic regions were analysed separately.
In this case and another (case 5), we also observed foci of highly cellular, viable tumour after imatinib treatment, similar to those seen in pretreatment biopsies but with dramatically reduced proliferative activity.
Hematoxylin and eosin-stained slides from these blocks were reviewed by two pathologists to identify the invasive breast carcinoma area, and the representative areas containing the most densely cellular viable invasive breast carcinoma tissue were chosen for coring.
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In order to exclude cellular debris, viable population was gated as P1.
Post-chemotherapy, cells can maintain viability by undergoing viable cellular responses such as cellular senescence, generating secretomes which can directly enhance the malignant phenotype.
In this regard, research specifically focused on interrogating the mechanisms and ability of tumour cells to circumvent and bypass apoptotic death mechanisms through adopting viable cellular fates such as cellular senescence and cytoprotective autophagy is warranted.
In specifically targeting the survival mechanisms that cancer cells can use to bypass death through apoptosis, viable cellular fates such as cellular senescence and cytoprotective autophagy could potentially be a target for impedance or manipulation and thus increase the death rate of cancer cells.
It arguably began with the attempts by Salmon and Hamburger to produce a viable cellular chemosensitivity assay in the 1970s, and continues to this day.
It showed that both microbial decay and hydrolysis of non viable cellular material proceeded at much slower rates as compared with biological systems sustained with substrate feeding.
The results indicate that commercial inkjet printing technology can be used to create viable cellular patterns with a resolution of 350 μm through the deposition of biologically active proteins.
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