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In the 1970s, a Berkeley biochemist named Bruce Ames devised a cellular test to do just that.
These heterobicycles behave as antioxidants by inhibiting the lipid peroxidation, and one compound (18b) is endowed with a significant anti-inflammatory effect in a cellular test.
The efficiency of such an exposure procedure with regard to stability and reproducibility depends on the optimal design at the interface between the cellular test system and the exposure technique.
A parallel cellular test was performed on the compound C1 whose IC50 is 5 µM (Table 2) and we observed that a 8 µM dose of C1, corresponding to 1.6-fold the IC50 of C1 on human GIIAPLA2 activity, does not decrease the stimulated PGE2 secretion by IL-1β at 1 ng/mL (data not shown).
The consideration of these adverse endpoints will dictate the selection of appropriate in vitro cellular test systems.
While human cellular test systems would overcome the problem of species specificity, in vitro cultured human immortalized cell lines do not represent normal cell types, and human primary cells cultured in vitro usually loose their tissue-specific functions.
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In addition to this, a lot of laboratories offer so-called cellular tests like the Lymphocyte Transformation Test (LTT) or the Elispot LTT and the CD 57 test.
Cellular tests have been applied to study the biocompatibility of high purity multiwalled carbon nanotubes (MWNTs).
Michigan Cancer Foundation-7 (MCF-7) cells, which are typical breast cancer cells, were used in all the cellular tests.
Fibroblast cells were chosen for cellular tests because they are a widely accepted model cell type used for cytotoxicity testing [70].
Therefore, further investigation of the potential toxicity, through cellular tests, is now warranted in order to provide a comprehensive health hazard assessment.
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