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The molecular patterns defined by oikosin gene expression establish the epithelium as an ideal and easily accessible monolayer cellular template for exploring coordinate regulation of gene expression, cell cell interactions involved in pattern formation, gene/genome amplification, and the role of temporal changes in nuclear architecture in regulating gene expression.
Briefly, double stranded cDNA was synthesized using 8 μg of total cellular template RNA according to standardized protocol (SuperScript double stranded cDNA synthesis kit; Invitrogen, Carlsbad, CA).
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The extraction of cellular 3D shape is of paramount importance for different purposes, such as to study volumetric deformation, quantify fluorescence expression in 3D, or generate cellular templates for 3D simulation models (Bassel et al., 2014; Yoshida et al., 2014).
However, whether a cellular chromosomal template can support read-through activation is not known.
The tail ends of cellular protein templates, regions often thought relatively inconsequential, may actually play a role in preventing normal cells from becoming cancerous.
However, for an unbiased detection and classification of all the different complexes in a cellular tomogram, template-free methods are needed (Xu et al., 2011).
However, as second-generation technology-based sequencing projects have become more cost-effective and feasible, a number of recent studies have exploited these methods to assemble plastome sequences from total cellular DNA templates.
We synthesized cDNA using Omniscript Reverse Transcriptase (Qiagen) using total cellular RNA as template.
Oligonucleotide inserts of targeted AAV library particles were amplified by nested PCR using whole cellular DNA as template.
The sequence ambiguities and gaps were resolved by dideoxy sequencing of PCR amplicons using total cellular DNA as template, and the genome sequences were deposited in GenBank.
Titanium-containing carbon nanosheets have been prepared from graphite powder, titanocene dichloride, pyrrole and other components by using mesostructured cellular foam (MCF) as template under microwave irradiation.
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