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Among the adenocarcinomas, no significant segregation was observed on this analysis that would suggest subclassification into distinct molecular subtypes that might be associated with differences in tumor or cellular staging criteria.
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Positive signals were observed in the cysts at all cellular stages of spermatogenesis (Fig. 1B).
scRNA-seq has demonstrated to be a powerful tool to identify and classify cell subpopulations [8], characterize rare or small subpopulations [9], and trace cells along dynamic cellular stages, such as during differentiation [10].
Maternal embryonic leucine zipper kinase (MELK) is a highly conserved serine/threonine kinase initially found to be expressed in a wide range of early embryonic cellular stages, and as a result has been implicated in embryogenesis and cell cycle control.
In conclusion, qPCR was a reliable technique to investigate viral load in in vivo and in vitro samples, and EHV-5 replication in equine epithelial cells may be influenced by cellular stages of differentiation.
This difference was evident in pre- and post-meiotic cellular stages, including spermatogonia, primary spermatocytes and round spermatids.
As in chickens and other vertebrates, zebrafish myogenesis proceeds through multiple cellular stages to produce the final complement of skeletal muscles [7].
However, to ascertain whether a modification in DNA synthesis occurred selectively in specific cellular stages in the two culture conditions, nuclear spreads were simultaneously immunostained with anti-BrdU and anti-Scp3 antibodies.
Both cellular stages share some common properties including stable cell-cycle arrest, flattened and enlarged morphology, increased cytoplasmic enzymatic vesicles, and changes in chromatin condensation and in gene expression patterns [49], [50].
We concluded from these results that reprogramming of fibroblasts to blood cells proceeds through an intermediate hemogenic endothelial cellular stage.
(A ) Plot of percentage of intron inclusion by intron in rank order for the three studied cellular stages.
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