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Like humans, congenitally deaf cats have an abnormal auditory nerve structure, with nerve endings that lack most of the small cellular spheres, called synaptic vesicles, that carry chemical transmitters from one nerve cell to the next.
The cells were further cultured until appearance of floating cellular spheres.
In contrast, floating cellular spheres (hence after designed as oncospheres) were observed in 49/55 tumor cultures (Figure 1 and Figure S2).
They revealed the unique ability of MGNTs to yield cellular spheres that could be amplified for very long periods.
The cellular spheres were dissociated, and plated at 200 or 1 cell/well in 96-well plates.
CD133- cells were also able to form floating cellular spheres with properties undistinguishable from those of CD133+ cells.
Similar(51)
Nor is such a capacity confined to the cellular sphere.
This basal faculty is represented across the cellular sphere, be it microbial or the mixed cellular ecologies that comprise multicellular eukaryotic organisms.
The abnormal cells underwent a transformation of the main cell body into sub-cellular spheres of ~5 µm in diameter.
Multi-cellular spheres consisting of 500 to 3000 cells (Fig 4A; top panel) grew from 11.4% of individually seeded CD133+ cells.
It was shown that rare, single-founder stem cells can form multi-cellular sphere structures under serum-free suspension conditions that are enriched for stem and early progenitor cells [ 17].
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