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Agents were, thereafter, quantitatively assessed for their ability to suppress the synthesis on TNF-α in a lipopolysaccharide (LPS -challenged mouse macrophage-LPS -challengedcreen, utilizing cultured RAW 264.7 cells.
To facilitate directed evolution of superfolded proteins, our initial goal was to develop a cellular screen for easily differentiating between cells expressing frGFP and sfGFP.
In this study we performed a cellular screen with FDA-approved drugs to identify necroptosis inhibitors.
MIRA-1 is a maleimide derived molecule identified along with PRIMA-1 in a cellular screen.
Aminopyrazoles were identified in a high-throughput cellular screen against P. falciparum asexual blood stages.
PfATP4 is the target of the spiroindolones, a novel antimalarial chemical class discovered in a cellular screen.
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Herein we discuss the potential impact of HT-HC cellular screens on medical neuroscience.
Our method provides patterned multicolor light sources at low-cost that are suitable for high-throughput microarray cellular screening.
This cellular screening will be useful for identification of human patients with β-galactosidase deficiency for chaperone therapy to be started in the near future.
The assay was validated using a panel of antivirals and proved to be a robust cellular screening assay for the identification of novel integrase inhibitors.
Therefore, a cellular screening assay was developed, which focused on integrase activity, where infection of MT4 cells with an HIV-1 based lentiviral vector was synchronized by temporary arrest at the reverse transcriptase step and subsequent release to enable integration.
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