Apatites restricted spreading and promoted the extension of cellular projections along the textured surfaces under confocal microscopy observation.
Because SICM can obtain non-contact (or force-free) images, delicate cellular projections were visualized on the surface of the fixed cell.
Confocal microscopic imaging of the actin cytoskeleton showed that cell morphology on two-dimensional substrates of fibrin showed marked changes, with higher thrombin concentrations producing cells with longer cellular projections.
We were interested in filopodia formation for DPSC growing on pSi samples, as this gives information about cell behavior since these cellular projections contribute to intercellular communication, cell adhesion, and motility[28].
Within the neuron phospho-synapsin was localized to cellular projections but not in cell bodies, as expected.
NOGO-B, ST14, Enolase 1, and ADAM15 had a notable presence within the cellular projections of the mesenchymal cells.
Additionally, the podocyte foot processes have important parallels with other dynamic actin-based cellular projections such as lamellipodia, filopodia, podosomes, and pseudopodia [42], [53].
On the other end of the spectrum MiaPaCa-2 and MPanc-96 clearly showed the mesenchymal characteristics of low cell-cell interactions and spindle like cellular projections.
Overexpression of Unc119 as a GFP fusion protein showed reduced actin polymerization and reduced formation of dendrite-like cellular projections (Figure 4B).
This rapid depolymerization and breakdown of actin determined that the stress fibers were no longer evident, and the F-actin staining was found diffuse throughout the cytoplasm and also in the long and thin cellular projections.
Hair cells are specialized epithelial cells equipped with bundles of actin filled, microvilli-like cellular projections called stereocilia, that transform mechanical sound signals into electrical ones and enable the sensation of hearing to occur.
