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Furthermore, our classifier makes use of the hierarchical organization of the KEGG database in three levels: at the top hierarchy there are the four branches 'Metabolism', 'Genetic Information Processing'Environmentaltal Information Processing' and 'Cellular Processes' (we do not consider 'Human Diseases' here).
Given the relatively recent discovery of miRNAs and their potential involvement in the regulation of almost all cellular processes, we examined whether miRNAs that are differentially expressed in CF airway epithelium control expression of ATF6.
To measure these cellular processes, we describe the use of a stable cell line expressing a fusion of green fluorescent protein (GFP) with IκBα that can be interrogated for either ubiquitination or phosphorylation using a unique set of terbium-labeled antibodies in a time-resolved Förster resonance energy transfer (TR FRET -based readouTR FRET -based
Given that BRCA1 is implicated in DNA damage-related cellular processes, we explored whether oxidative stress regulates the BRCA1 and Smad3 interaction.
In order to understand how the ALS8-associated mutation affects VAP function and other cellular processes, we used a gain of function approach.
To examine the downstream effect of Sirt1 deacetylase activity in cellular processes, we measured the expression of both total and acetylated p53 in aging rat tissue.
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To remove GO terms that were too broadly defined (e.g., "biological process" or "cellular process"), we excluded GO terms to which 500 or more Drosophila genes had been assigned.
To examine whether the difference in efficiency of invasion of keratinocytes when compared to endothelial cells is due to the bacteria utilizing a different cellular process, we measured the internalisation of S. aureus FnBPR1 11 by HaCat cells pre-treated with inhibitors of cell function used previously in studies with endothelial cells [17].
In keeping with a central role of mitochondria in this cellular process, we demonstrated that mitochondrial dehydrogenase activity was significantly reduced, a finding that is consistent with mitochondrial dysfunction as a consequence of the oxidative conditions.
Although TF and PTM bring about regulatory mechanisms at different phases of cellular process, we have combined TF and PTM and refer to them as simply regulators in order to capture the maximum regulatory apparatus in eukaryotes. 5.
For the 17 modules that contain groups of genes enriched in the same cellular process, we found that the regulatory functions of the 76% (26/34 counting multiplicity) of the TFs are consistent with one of their modules' over-represented functional categories (see Figure 2).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com