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The recently developed rapid one step (i.e., <4 hr) ferumoxides-protamine sulfate method was used to label the CTLs and generate an effective cellular probe compatible with MRI [38].
This possibility was evaluated using 17C-dhSph as a cellular probe to evaluate the activity of sphingosine kinases.
Nevertheless, we have achieved sufficient potency, and further gains in selectivity should lead to a cellular probe of autophagy.
In that regard, the ACPP technology might offer an advantage over the detection of passively diffusing Gd, as it would allow local signal enhancement by cellular probe uptake, specifically in areas with increased proteolytic activity.
Although this compound shows limited utility as a cellular probe of PAD activity, its discovery does demonstrate that it is possible to identify potent non-active-site-targeted reversible PAD inhibitors.
Due to its carboxyl group, GSK-J1 does not have potent cellular activity, but the ethyl ester prodrug, GSK-J4, has been shown to be an effective cellular probe.
Unlike spots containing control DNA, blank spots are not representative of non-specific hybridization to cellular probe DNA, do not behave well as control spots, and should be disregarded for threshold detection on custom arrays.
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The use of these complexes as cellular probes and imaging reagents has also been discussed.
"Fluorescent protein voltage sensors are recombinant proteins that are designed as genetically encoded cellular probes of membrane potential using mechanisms of voltage-dependent modulation of fluorescence.
An improved, non-functionalized route to plasmonic nanoparticle based cellular probing through osmolyte mediation.
Further improvements in cytologic specimen preservation and the development of multiparametric analysis using other cellular probes of neoplasia could substantially augment the utility of this diagnostic technology.
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