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Pure DNA from the cellular portion of maternal blood and fetal cord blood was extracted with QIAamp Mini Blood Kit (Qiagen).
This pattern contrasted with the cellular portion of the venom gland transcriptome, where there was a negative relationship between protein abundance and evolutionary rate, similar to that found in most organisms [ 116, 117].
This allowed us to compare the relative age of a COG (the percentage of bacterial genomes that have a particular COG) to the genomic or cellular portion of this dataset that COG composes.
In comparison, the exact function of the leucine rich-repeats (LRR) region located before the 70-amino acid island domain in the extracellular cellular portion of BRI1 has not yet been described, due to a lack of specific mutant alleles.
The cellular portion of the mass contained sheets and nests of epithelioid cells with moderately light basophilic cytoplasm, round to oval finely stippled central nuclei and 1 4 prominent purple nucleoli.
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IF stainings of atherosclerotic lesions derived from the common carotid artery are shown in Figure 1 C, and immunostainings provided in Supplementary material online, Figure S1 show the extent and orientation of the positive areas with abundant FPR2/ALX expression in highly cellular portions of the plaque and in the vicinity of the necrotic core (see Supplementary material online, Figure S1 ).
Glioma cell lines were plated on glass coverslips or in coverglass chamber slides (Lab-Tek, Nunc) and stained in vivo for 30 min at 37°C by 2 nM QD-EGF or 5 µg/ml MAb against the extra-cellular portion of Her1 followed by either conventional fluorophore labeled GAMIG or QD-coupled GAMIG.
Following ACTH injection, c-Fos immunoreactivity was quantified in key appetite-associated hypothalamic nuclei including the ventromedial hypothalamus (VMH), dorsomedial hypothalamus, lateral hypothalamus (LH), arcuate nucleus (ARC) and the parvo- and magno-cellular portions of the paraventricular nucleus.
Cellular portion was resuspended in PBS.
Only half the chapters, those dealing with the big ideas at the molecular, cellular, and a portion of the organismal scale, were used in this study (see the complete reading schedule online at: www.bio.davidson.edu/people/macampbell/111/Bio111reading_F2010.html).edu/people/macampbell/111/Bio111reading_F2010.html
Cholinergic neurons, revealed either by ChAT and/or p75NTR immunochemistry, were predominantly located medially and ventrally in the cellular, less dense portion of the NB (Fig. 5C and E).
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