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Regenerative engineering scaffolds provide mechanical support and nanoscale control over architecture, topography, and biochemical cues to influence cellular outcome.
We also performed in silico mutagenesis analysis to characterize the effects of oncogenic mutations on the negative regulators and to observe the cellular outcome (whether there is/is not inflammation).
With an FC coating, micropillars induced a better cellular outcome as the cells had the greatest circularity, smallest cell area and highest Na+/K+-ATPase and ZO-1 gene and protein expression.
Hydrodynamic analysis of these data, combined with data from studies of 6 ns microbeam irradiation, demonstrates the primacy of shear-stress impulse in determining cellular outcome resulting from pulsed laser microbeam irradiation spanning a nearly two-orders-of-magnitude range of pulse energy and pulse duration.
Though flies and humans achieve this transcriptional program through the activation of different genes, the cellular outcome of the transcriptional profile remains conserved.
This finding underlined that certain genes are regulated upon IR exposure irrespective of time of day, but that the quality of induced regulation is influenced by circadian oscillation of gene expression, which may have consequences for cellular outcome.
In the threshold model the amount of p53 determines cellular outcome [27].
Several models have been proposed to explain how p53 determines the cellular outcome.
Often different post-translational modifications occur in tandem, and the cross talk between these modifications determines the final cellular outcome.
For instance, it is possible that when two TFs are expressed together in the same single cell they lead to a certain cellular outcome.
In contrast to the similar occupancy of binding sites, cell cycle analysis and expression profiles show a marked difference in cellular outcome.
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