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He used regenerating tail tissue of the tadpole for his observations, and shows that cellular organisation may be studied here with results which tissue cultures cannot provide.
Even though these results suggest that specific targeting of the CSC population might not be a useful therapeutic strategy, research into the hierarchical cellular organisation of malignancies has provided many important new insights in the biology of tumours.
A number of studies using more modern techniques (such as immunohistochemistry, in situ hybridisation, axonal tracing, single cell labelling, heterotopic and orthotopic grafting, and the manipulation of gene expression by electroporation) have shown that the hindbrain has a truly metameric cellular organisation.
Co-culture of Calu-3 cells with Wi38 lung fibroblasts was achieved on the scaffold to create a submucosal tissue analogue of the upper respiratory tract, validating CHyA-B as a platform to support co-culture and cellular organisation reminiscent of in vivo tissue architecture.
Although the repair tissue was not hyaline cartilage, it did show cellular organisation and proteoglycan deposition similar to hyaline cartilage without any evidence of angiogenesis.
No obvious changes in morphology or cellular organisation occurred in these organisms over the next few billion years.
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Tumours in vivo represent complex three-dimensional cellular organisations with a characteristically harsh microenvironment comprising high cell density, low pH, toxic metabolites, low oxygenation and elevated interstitial pressure.
Within the GI tract GLP2 has significant intestinotrophic effects involving diverse cellular organisations despite the finding that GLP2 receptors are mainly localized on enteric neurons, endocrine cells and subepithelial myofibroblasts [ 39].
In addition, the possibility of generating cell polarisation simply via pattern topology may be useful for studying mechanisms underlying cell polarisation, but also responsible for maintaining sub-cellular organisation and architecture.
One of the difficulties in constructing spatiotemporal models on tissue scale is that important components such as material parameters of individual cells may affect the multi-cellular organisation and hence have to be included in the model.
Several reports have shown that the sub-cellular organisation of cells [16] and the orientation of the mitotic spindle [14] are directed by the topology of the ECM on which cells spread.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com