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This new imaging technology offers specific characteristics: a way to gain access to organs and tissues in vivo, sensitive detection of fluorescent signals, and cellular observations with rapid acquisition at near real time.
Cellular observations were then confirmed in ex vivo fetal intestinal xenografts.
Further studies are needed to relate these cellular observations to disease phenotype in a large group of patients.
Accordingly, to further confirm the cellular observations in an ex vivo model of human intestinal development, we determined the effect of cortisone on the expression of selective innate inflammatory response genes in human intestinal xenografts.
In previous publications using fetal intestinal xenografts [7] [9], [15], we have been required to confirm isolated cellular observations in selected samples by repeating the observation in fetal intestinal xenografts, thought to be a more physiologically relevant ex vivo model.
Importantly, further cellular observations confirmed that these tubules extended from the cis-Golgi.
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This cellular observation in lung tissue did not correlate with a similar increase in peripheral blood analysis.
DOI: http://dx.doi.org/10.7554/eLife.05635.001 Like so many model organisms, the zebrafish was chosen for its tractability and, especially, its potential for genetic analysis and cellular observation.
Cellular localization observations were performed on the LSM510 confocal microscope (Carl Zeiss, Germany).
These results suggest a natural methodology to distinguish the different genetic activation/inactivation rates in the cellular phenotype observations, which is described in detail in the continued text.
Differences in these growth form traits can be investigated in Aristolochia species of interest, including A. fimbriata, beginning with cellular level observations and descriptions of development of secondary growth ("wood").
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CEO of Professional Science Editing for Scientists @ prosciediting.com