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The cybernetic perspective relies on proposing "mechanisms" of choice behavior by which biological systems drive enzyme syntheses and allosteric processes for chemical reactions that promote perceived local or global cellular objectives.
Their study finds that minimal reaction sets are strongly dependent on medium constituents and cellular objectives.
EFMs represent the various independent pathways available for the cell to achieve its cellular objectives [ 17].
Not every candidate solution identified by the qualitative approach would meet the cellular objectives.
Such an assumption, however, is likely to be invalid for individual cell types in multicellular organisms, where cellular objectives may differ greatly both between and within tissues.
Indeterminate reactions (IRs) primarily consist of substitutable reactions i.e. reactions which can be substituted with other reactions to achieve the cellular objectives.
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Z represents the cellular objective function of all the metabolic reactions where the relative weights are determined by the coefficient c j. S ij corresponds to the stoichiometric coefficient of the metabolite i involved in reaction j.
A recent work by Ranganathan et al., addresses this issue by suggesting a novel method, OptForce, that does not rely on a definition of a cellular objective function, though it requires additional experimental flux measurements as input [23].
An underlining assumption of FBA is that the cellular objective is the maximization of biomass growth.
The cellular objective was assumed to be the maximization of biomass production.
ATP-production was implemented as a cellular objective by introducing an artificial reaction that consumes cytosolic ATP.
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