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Nonetheless, characterizing the 2-D motion at high time-resolution can serve as the basis of modeling cellular motion in natural environments.
Cellular motion was first tracked and velocity calculated as described previously.
If changes in expression patterns occur consistently in many genes, this might indicate a collective cellular motion in the embryo.
In this model, food is converted into diffusible molecules, cellular motion as well as stored energy that ultimately fuels division of the cells.
Cellular motion was detected by exciting the dye using an exposure time of 50 msec at a laser excitation wavelength of 485 nm and detecting emission at 535 nm on an Olympus IX81 confocal time-lapse microscope.
The cytoskeleton, a complex network of protein fibers in eukaryotic cells, provides a dynamic structural framework that is crucial for maintaining normal cell activity, including cell shape, cellular motion, division, and intracellular transport among other processes [ 1- 3].
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Advancements in imaging technologies, genetic recombination techniques, laser ablation, and microfabricated tissue models have enabled quantitative descriptions of the cellular motions and tissue deformations and stresses with unprecedented temporal and spatial resolution.
We obtained theoretical trajectories of cellular motions from numerical simulations and calculated the corresponding statistical quantities.
Finally, it remains possible that the two are not causally related, but that the tissue morphogenetic movements merely create a moving platform in which the cellular motions take place.
Diffusion-weighted magnetic resonance imaging (DW-MRI) is a technique used to reflect the microstructural properties of tissues, related to the intra- and extra-cellular motion of free water molecules, indicative of tissue cellularity and structure.
To accurately deduce diffusion coefficients using RICS in live cells, one has to account for and subtract slow background motion (gross cellular motility) and movement of large visible vesicles or organelles that would give rise to fluctuations with an apparent diffusion of <0.002 μm/s.
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