Cells were cotransfected with EGFP as a cellular marker (green), and treated with the cell permeable infrared nuclear dye Draq5 (blue).
Primary rodent cortical neurons were transfected with vectors encoding the cellular marker mApple, and either Htt96Q-EGFP or EGFP alone, treated with KPT-335 or -350, and imaged by automated microscopy (Fig. 3a).
This enabled us to independently track the survival of neurons using mApple as a cellular marker in the red channel, and the expression and subcellular distribution of TDP43-EGFP in the green channel.
Moreover, overexpression of miR-128 led to decreased expression of SMC cellular marker proteins, such as smooth muscle actin (SMA) and calponin, in TGF-β1-induced SMC differentiation.
Thanks to its periodic expression, this putative orphan nuclear receptor could serve as a suitable cellular marker for studying changes of epidermal cell sensitivity to 20E during the cell cycle.
Using immunohistochemistry, we showed that B2 increased c-Fos expression, a cellular marker for neuronal activity, in the ventrolateral preoptic (VLPO) area, a sleep center in the anterior hypothalamus.
The Ki67 protein is a well-known cellular marker strictly associated with cell proliferation.
Thus, with Ttk detection used as a cellular marker, 60 minutes was the minimal time required to identify a cell transformation after ectopic N-activation.
If these different models all confirm that this ganglioside is altered when GNE is mutated, GM3 levels could then be tagged as a cellular marker of HIBM.
Statistical comparison was performed using one-factor analysis of variance (ANOVA) with transcription factor, cellular marker, genotype or time as variables, followed by post-hoc analysis when significant differences were observed, using Statview 5.0 software (SAS institute Inc).
Ki67 is a cellular marker for proliferation.
