In addition to the cellular layers, the integument often includes a noncellular coating, or cuticle, that is secreted by the epidermis.
At this stage, a zebrafish embryo has three distinct cellular layers: the enveloping layer (EVL), deep cell layer (DEL) and yolk cell, which can be subdivided into the yolk cytoplasmic layer (YCL) and yolk syncytial layer (YSL) adjacent to the EVL.
During linear heart tube and looping stages, the heart wall is composed of only two cellular layers, the myocardium and endocardium.
By P11, when control cortex had substantially thickened with well-defined cellular layers, the EmxKO cortex had further degenerated with additional loss of tissue integrity.
Histologic examination (Supplementary Figure S1a) showed that photoreceptors, inner retinal cells and ganglion cells were distributed in three cellular layers, the outer nuclear layer, inner nuclear layer and ganglion cell layer, respectively, in WT retinas.
As the first cellular layer of the human cornea, the corneal epithelium plays an important role in the evaluation of corneal remodeling after refractive surgery [ 14].
As previously reported for the MOB (Bhalla and Bower, 1997; Kay and Laurent, 1999; Rinberg et al., 2006; Doucette et al., 2011), large well-isolated spikes were found exclusively when the electrode tracks traversed the external cellular layer of the AOB.
The buffy cellular layer at the interface was collected and washed three times (centrifugation 300×g, 10 min, 4 °C) in RPMI-1640 medium (Gibco/BRL, USA), then cells were resuspended in complete RPMI-1640 medium and counted.
The outer cellular layer of the cap cuticle is made of bladder-shaped, thick-walled hyphae, each individually supported by a small stalk that extends down into a "gelatinized zone".
A dense cellular layer of the pNr envelops huge myelinated fiber bundles of the superior cerebellar peduncle.
