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Mapping the information above onto pathways or cellular interaction networks provides an additional informative view of therapeutic intervention points on the pathway: for example, for combination studies.
Datasets describing cellular interaction networks need to be integrated into predictive models of in vitro stem cell development.
Cellular interaction networks can be used to analyze the effects on cell signaling and other functional consequences of perturbations to cellular physiology.
Based on these data, the current study shows that tumor spheroids represent a novel tool to decipher determinants of tumor immune escape and to study cellular interaction networks in 3D.
Single tumor spheroids can be used i) to monitor tumor growth, ii) to investigate immunosurveillance and associated immune escape by soluble mediators, iii) to investigate tumor spheroid infiltration and spheroid destruction, and iv) to analyze cellular interaction networks by fluorescence microscopy and flow cytometry.
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It has been found that TCs present morphological contact with immune cells to form a cellular interaction network to participate in immune regulation [ 19].
Given that about 25 000 protein-coding genes in the human genome can occur in multiple splice forms, the number of genes integrated in our cellular signaling model is still a fraction (ca. 2.5%) of the overall cellular interaction network; moreover, it is as yet devoid of aspects of the immune system and metabolism, such as cytochrome c, as well as the human microbial milieu.
Inter-cellular interaction networks such as neuronal networks and immune responses determine organ and organism-level function.
It fueled proteome-wide studies leading to the establishment of intricate cellular protein interaction networks.
Table 2 shows the comparison of network parameters of SARS to KSHV as well as other cellular protein interaction networks.
Specifically, we suggest that peptide recognition modules, such as PDZ, SH3, SH2, WW, EH and other domains, which show both selectivity and promiscuity in their interactions [3], [19], [24], [38], function as adaptive molecular "synapses" of cellular protein interaction networks, rather than perform as Lego Block-like elements for assembly of pre-defined and immutable structures.
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