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The objective of this study was to demonstrate that the silica-coated CdSe QDs can be used as labelling agents for long-life cellular imaging, cancer cellular imaging using cLSM and fluorescence microscopy.
Firstly, partial d→f energy transfer following two-photon excitation of the IrIII unit allowed near-IR excitation to generate both Ir-based (μs timescale) and Eu-based (ms timescale) emission components, which can be used independently as the basis of cellular imaging using time-gated detection.
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Molecular and cellular imaging strategies using SPECT- or PET-based approaches have great potential to fulfil these requests of allograft surveillance.
This new approach to cellular imaging by using dual d/f emitters should therefore enable autofluorescence-free sensing of two different analytes, independently, simultaneously and in the same regions of a cell.
The above cellular imaging protocol used for Tat peptide-conjugated PS-PEG micellar QDs was adopted, with the main modification being that the concentration used for RGD peptide-conjugated micellar QDs was 100 nM (QDs in cell culture medium).
Cellular ATP dynamics were measured on single-cell imaging using the LV200 imaging system as described previously [ 22].
Cellular proliferation rates were analysed by live-cell imaging using the IncuCyte system (Essens Bioscience, Birmingham, UK).
The progression of cellular structures from the onset of cells through extinction was analyzed by flame imaging using an intensified CCD camera.
By exploiting specific 'inducible' molecular targets, or cellular events in diseases, molecular imaging uses targeted imaging agents to generate the image contrast [6].
Images were analysed with the MetaXpress cellular imaging analysis software using the cell scoring module.
High-content cellular imaging cytometry was used to analyse the expressions of TNF-α, NF-κB p65, and NF-κB p50.
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