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Use of the Ingenuity Pathway Analysis software identified 47.7% (104/218) of genes as falling within five molecular and cellular function groups; cell morphology, cellular movement, cellular development, cellular growth and proliferation, and cellular assembly and organization.
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We applied an innovative method in which we tested for the effect of groups of genes defined according to cellular function (functional gene group analysis).
On the basis of molecular and cellular function, major groups of detected proteins included cellular growth, proliferation, survival, cellular assembly, and organization (Table S3).
We refer to grouping genes according to cellular function as "horizontal grouping," as opposed to the classical grouping of genes in pathways, which we refer to as "vertical grouping".
These pathways and genes represent a small number of related cellular functions, so we grouped them in functional groups.
Clustering of these genes according to their cellular function revealed that the most representative group was that of signal transduction molecules followed by groups of genes involved in cell adhesion and motility process (array supplementary data).
One group was formed of remaining genes that are known to be expressed in the synapse but do not share any cellular function with other genes (this gene group was called "unknown").
The findings from these studies can be broadly grouped into categories: cellular function and metabolism; dysregulation and risk for malignancy; intercellular and systemic effects; cell morphology and differentiation; enzyme effects; pharmacologic effects.
In addition, we identified genes expressionally altered during MPA-induced β-cell death and grouped these genes by cellular function.
The 55 and 129 genes were grouped into categories based on their cellular function using Gene Ontology (GO).
As (III) reacts with the sulfhydryl groups of enzymes and proteins, thereby inhibiting cellular function and resulting in death [ 7].
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