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Analysis in the cellular function category revealed major clusters in synaptic and signaling functions (Fig. 7A).
Indeed, there were prominent clusters of transcription and translation in the cellular function category that might have roles in differentiation and morphogenesis.
Across the cellular function category, the most abundant terms were "cellular component" (7,620 for C4, 7,759 for CAV), "cell" (7,580 for C4, 7,725 for CAV), "organelle" (5,589 for C4, 5,735 for CAV) and "macromolecular complex" (1,772 for C4, 1,829 for CAV). Figure 3 summarises level 1 and 2 GO annotation of unigenes.
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The core rickettsial protein distribution across cellular function categories is also similar to another obligate intracellular pathogen, Chlamydia trachomatis, suggesting that this lifecycle is defined by reduction of many genes with conserved cellular functions (save translation) in facultative intracellular (Yersinia pestis) and extracellular (Escherichia coli) pathogenic bacteria.
Analyzing a crude depiction of the R. felis proteome, Ogawa et al. [40] reached a similar observation as their 172 identified proteins sorted into cellular function categories similar to those assigned for our core proteins, although with far fewer members per category (Figure 8B).
Other cellular function categories showing a high degree of change include cancer, cell morphology, cell death, cellular assembly and organisation, and molecular transport.
Furthermore, the number of genes assigned to each of the top five molecular and cellular function categories between time points 1 h and 24 h was higher in E. coli inoculated cells compared to S. aureus inoculated and control cells.
In the 'Molecular and Cellular Functions' category 'cell death and survival'cell-to-cellcell signaling and interaction' , and 'cellular growth and function' were the most affected.
Westar leaf expression had been found to be meaningful in an earlier comparison of transcripts from extremely hairy leaves of B. villosa using microarray analysis [ 34], genes with this level of relative expression or greater were categorized into 35 sub-cellular function categories or sub-categories using Mapman [ 45].
Cell and cell parts were dominant groups in the "cellular component function" category.
The other enriched functional categories, cellular function and distribution, as well as processes and functions that were either activated or suppressed in each of the three groups are all summarized in Table 3.
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