Sentence examples for cellular fixation from inspiring English sources

We then performed hierarchical clustering to demonstrate whether cellular fixation alone could change gene expression.

This established protocol allows for a shorter infection time, a more synchronized infection, and earlier cellular fixation periods, while maintaining the same Salmonella intracellular growth and trafficking profiles observed with longer and more commonly used infection protocols (Figure S3A-C) [56].

Cellular fixation and permeabilization are necessary for antibody-mediated detection of intracellular antigens by FCM.

Cellular fixation was followed by washing twice with PBS and a 10 min treatment with 0.1% Triton‐X 100 in PBS.

It is therefore less bulky than tags such as GFP, and offers several other technical advantages.[ 21] The coupling of specific fluorophores or isotopes to the UAAs takes place only after cellular fixation, when functional perturbations are no longer a concern.

The nine CCM-related GOIs that were tested are representative of all known CCM complexes in Trichodesmium: the carboxysome (ccmM, ccmK2, ccmK3) that contains the cellular Ci fixation enzyme RubisCO (rbcL; [19]), HCO3− transporter named BicA (bicA1, bicA2), and the specialized NADPH dehydrogenase NDH-I4 (ndhD4, ndhL4, ndhL, and chpX).

Cellular debris, fixation artifacts and doublets were gated out with FL2-area and FL2-width parameters.

One consequence of the engineering of a high-capacity carbon sink in cyanobacteria, however, has already been noted, i.e. the increased rate of cellular CO2 fixation [ 6, 29, 30].

Other potential drawbacks of a fixed endpoint immunofluorescence assays include the cost of antibodies as well as potential loss of weakly adherent mitotic cells and distortion of cellular structures during fixation.

A variety of chemicals show selective herbicidal activity, with many inhibiting the light-powered electron transfer used by plants to generate the NADPH and ATP that support carbon fixation, cellular growth and division, the mode of action being competitive inhibition of quinone reduction and protonation by the Photosystem II (PSII) reaction centre (Draber et al., 1991).

Collected cell pellet was fixed by adding 1 mL of 2.5% glutaraldehyde that was buffered in 0.1 M phosphate buffer saline (PBS) of pH = 7.4 (for fixation of cellular protein content, and to stop all culture reactions), and was cooled at 4°C for 2 h.